Contact an Account ManagerDetermination of acetylcholine and choline in rat brain
Summary
Type: HPLC
Application #: 102240
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: electrochemical: +500mV versus Ag/AgCl
Conditions: eluent: 0.091M Na acetate pH 5.0, 25mg/l Na octyl sulphonate, 1.2mM tetramethylammonium chloride, 10mM Na2EDTA,
flow rate 0.8ml/min,
temperature 37°C.
Authors: Asano, M.
Source: J Liquid Chromatogr 9 (1986) 199 - 215
Details
Legend: Chromatogram of a standard mixture.
Ch = choline,
EHC = ethylhomocholine (int. std.),
ACh = acetylcholine,
200pmol each injected.